From tmatsungo at science.uz.ac.zw Tue Feb 20 01:52:03 2007 From: tmatsungo at science.uz.ac.zw (Mr Tonderayi Matsungo) Date: Mon Feb 26 12:16:44 2007 Subject: [rat-forum] Help with Small scale rat pelleting techniques and alternative feeds Message-ID: <20070220095203.er0imksby88cc8w4@webmail.uz.ac.zw> Good Day all! RE:Help with Small scale rat pelleting techniques and alternative feeds We want to work with rats in a feeding (Obesity) study and would like to supplement the diet with powdered supplements and make pellets. This is proving difficult and we have apporoached animal feed companies and they could not help us since they only work with batches starting form one Tonne upwards. Could anyone help us with pelleting techniques andgeneral protocol that we can use in our lab (small scale)to produce pellets. Better still we would like to know whether there are other methods besides pelleting that can help us deliver the supplliments to the rats (dietary intake). Regards, Tonde matsungo Mr Tonderayi Matsungo Department of Physiology College of Health Sciences University of Zimbabwe P.O.Box MP 167 Mt Pleasant, Harare Zimbabwe Occupation:Nutritionist/Physiologist/Researcher Phone:263 4 303211 Ext 1057 Fax: 263 4 333678 Cell: 263 91 955 466 Alternative Email: tmatsungo@scientist.com ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From jean.harris at stonebow.otago.ac.nz Fri Feb 23 23:55:14 2007 From: jean.harris at stonebow.otago.ac.nz (Jean Harris) Date: Mon Feb 26 12:16:44 2007 Subject: [rat-forum] Help with Small scale rat pelleting techniques and alternative feeds In-Reply-To: <20070220095203.er0imksby88cc8w4@webmail.uz.ac.zw> References: <20070220095203.er0imksby88cc8w4@webmail.uz.ac.zw> Message-ID: <4EAB2A43-85A5-4D9A-B99E-DE3FCFDB3970@stonebow.otago.ac.nz> While I can't help you with pelleting, I can make a comment that might be relevant and of interest to you. We crossed the New Zealand Genetically Hypertensive (GH) rat with the Brown Norway strain to look for markers associated with heart mass and blood pressure in the F2 generation: http://tinyurl.com/2bpg2g After performing this cross we became aware on an additional phenotype of internal elastic lamina lesions in the aortas of the normotensive BN strain. We performed another cross theoretically identical to the first cross to Identify the genomic region(s) responsible for the lesions: http://tinyurl.com/2zgyv4 Though we were of course aware that phenotypic impacts can vary depending on the environment, we were still surprised to see the extent of variation between the two crosses. The phenotypic variation in the IELL phenotype was particularly evident between F1 rats raised in on campus facilities and F1 rats raised at the Department of Laboratory Animal Sciences. I spent some time trying to determine the reason for the variation and think it was due to the fact that DLAS autoclaved all the food they gave our rats whereas the rats raised on campus were given rat chow straight from the sacks it came in. The IEL lesions in F1 rats were far more severe in the DLAS-raised rats than in rats raised on campus. I think this may have been due to the destruction of nutrients in the rat chow when it went through the autoclave. It would be analogous to Professor John Rapp feeding all his F2 rats a high salt diet to bring out the full phenotypic expression of any genetic tendency to hypertension. Anyway, when I mentioned this to the head of DLAS he made the astonishing reply that they knew autoclaving destroyed nutrients so they put in twice as many nutrients before the rat chow was put through the autoclave. The mind boggles. Anyway, I had no way of taking this matter further so I dropped it. But I do think that anybody who feels they have to autoclave rat chow should be aware that the autoclaving process will destroys many nutrients. Regards, Jean Harris On 20/02/2007, at 8:52 PM, Mr Tonderayi Matsungo wrote: > Good Day all! > > RE:Help with Small scale rat pelleting techniques and alternative > feeds > > We want to work with rats in a feeding (Obesity) study and would > like to supplement the > diet with powdered supplements and make pellets. This is proving > difficult and we have > apporoached animal feed companies and they could not help us since > they only work with > batches starting form one Tonne upwards. > > Could anyone help us with pelleting techniques andgeneral protocol > that we can use in our > lab (small scale)to produce pellets. Better still we would like to > know whether there are > other methods besides pelleting that can help us deliver the > supplliments to the rats > (dietary intake). > > Regards, > > Tonde matsungo > > Mr Tonderayi Matsungo > Department of Physiology > College of Health Sciences > University of Zimbabwe > P.O.Box MP 167 > Mt Pleasant, Harare > Zimbabwe > Occupation:Nutritionist/Physiologist/Researcher > Phone:263 4 303211 Ext 1057 > Fax: 263 4 333678 > Cell: 263 91 955 466 > Alternative Email: tmatsungo@scientist.com From p.sloane at nuigalway.ie Fri Feb 23 15:17:08 2007 From: p.sloane at nuigalway.ie (Sloane, Peter) Date: Mon Feb 26 12:16:44 2007 Subject: R: [rat-forum] Help with Small scale rat pelleting techniques and alternativefeeds References: <20070220095203.er0imksby88cc8w4@webmail.uz.ac.zw> Message-ID: Tonde, I have successfully used daily gastric lavage to dose rats with a pharmocolgical agent. Using a short period of Isoflurane by face mask allows reproducible and accurate dosing of any material / agent into the stomach. This could equally well be applied to a dietary supplement. Should you require any further details please let me know. Peter Sloane. REMEDI, NUI Galway, Ireland. p.sloane@nuigalway.ie -----Messaggio originale----- Da: rcf-bounces@rgd.mcw.edu per conto di Mr Tonderayi Matsungo Inviato: mar 20/02/2007 07:52 A: rcf@rgd.mcw.edu Oggetto: [rat-forum] Help with Small scale rat pelleting techniques and alternativefeeds Good Day all! RE:Help with Small scale rat pelleting techniques and alternative feeds We want to work with rats in a feeding (Obesity) study and would like to supplement the diet with powdered supplements and make pellets. This is proving difficult and we have apporoached animal feed companies and they could not help us since they only work with batches starting form one Tonne upwards. Could anyone help us with pelleting techniques andgeneral protocol that we can use in our lab (small scale)to produce pellets. Better still we would like to know whether there are other methods besides pelleting that can help us deliver the supplliments to the rats (dietary intake). Regards, Tonde matsungo Mr Tonderayi Matsungo Department of Physiology College of Health Sciences University of Zimbabwe P.O.Box MP 167 Mt Pleasant, Harare Zimbabwe Occupation:Nutritionist/Physiologist/Researcher Phone:263 4 303211 Ext 1057 Fax: 263 4 333678 Cell: 263 91 955 466 Alternative Email: tmatsungo@scientist.com ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ rcf mailing list rcf@rgd.mcw.edu http://gray.hmgc.mcw.edu/mailman/listinfo/rcf -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: application/ms-tnef Size: 3849 bytes Desc: not available Url : http://gray.hmgc.mcw.edu/pipermail/rat-forum/attachments/20070223/c44e77bc/attachment.bin From veronique.malard at cea.fr Fri Feb 23 02:45:06 2007 From: veronique.malard at cea.fr (=?iso-8859-1?Q?MALARD_V=E9ronique?=) Date: Mon Feb 26 12:16:44 2007 Subject: [rat-forum] Rat EGF Message-ID: <3789CD0DE868404E9070AA3F4109F95EB4F983@YACA.intra.cea.fr> Hi Two years ago you asked ; Does anyone know of a commercially available ELISA or RIA for rat EGF? I am still looking for the same.... Did you find a kit or an antibody ?? how did you manage?? Thanks a lot for your answer Best regards V?ronique MALARD veronique.malard@cea.fr CEA/DSV/iBEB/SBTN/LBSP BP 17171 30207 Bagnols/C?ze cedex FRANCE T?l : 04 66 79 68 18 Fax : 04 66 79 19 05 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://gray.hmgc.mcw.edu/pipermail/rat-forum/attachments/20070223/e4c63cc6/attachment.html