[rat-forum] Rat sperm cryopreservation

[Sztein, Jorge (NIH/NIAID) [E]]

There was a paper published in BOR November 26 2008 By Yasunari Seita.
Also at the MGI list not long ago we have an answer on the same question:


The National Bio Resource Project for the Rat in Japan (NBRP-Rat) is one of the largest worldwide resource center, collecting, preserving and supplying of unique rat strains. We have collected over 500 rat strains, characterizing their phenotypes and genotypes, cryopreserving their embryos and sperm, and distributing the collected rat strains to researchers.

http://www.anim.med.kyoto-u.ac.jp/NBR/Default.aspx



We have successfully recovered rat frozen sperm by AI (Artificial

Insemination) and ICSI. We have also made a DVD protocol for cryopreservation of embryos and sperm. If you were interesting in our technologies, please contact us via website.

http://www.anim.med.kyoto-u.ac.jp/NBR/contact.aspx



Tomoji Mashimo,

Akiko Takizawa,



********************************************************

Tomoji Mashimo

Institute of Laboratory Animals

Kyoto University Graduate School of Medicine, Yoshidakonoe-cho Sakyo-ku, Kyoto 606-8501 JAPAN

Tel: +81-75-753-9318  Fax: +81-75-753-4409

E-mail: tmashimo at anim.med.kyoto-u.ac.jp<mailto:tmashimo at anim.med.kyoto-u.ac.jp>

In my lab, if that can help you, we freeze embryos, and works really well.
Cheers,
Jorge

Jorge M Sztein, DVM, PhD,
Associate Program Director
CMB Cryopreservation and Assisted Reproduction,
NIH, NIAID,
TwinBrook II, Room 201 D
12441 Parklawn Dr.
Rockville, MD 20852
PH 301-594-5809
Fax 301-435-8908
szteinj at niaid.nih.gov<mailto:szteinj at niaid.nih.gov>

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From: rat-forum-bounces at mcw.edu [mailto:rat-forum-bounces at mcw.edu] On Behalf Of diane.h.hawkins-brown at gsk.com
Sent: Tuesday, March 03, 2009 1:45 PM
To: rat-forum at mcw.edu
Subject: [rat-forum] Rat sperm cryopreservation


Does anyone have information regarding the freezing down of rat sperm and whether the technique is similar to freezing mouse embryos?
Diane

Diane Hawkins-Brown
Study Delivery Team Leader
LAS, RTP, B6L4
Glaxo SmithKline
diane.h.hawkins-brown at gsk.com
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